Glutathione, Tylenol & Your Health: What You Should Know When Good Drugs Go Bad: Acetaminophen Toxicity tl;dr pharmacy Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Sex differences in glutathione metabolism and acetaminophen toxicity Here's how to make Tylenol work way better Dose it correctly: 1,000 mg at a time, spaced every 8 hours. Smaller doses don't control pain as well. Take it