Acetaminophen Depletes Glutathione Essential Formulas The biochemistry of acetaminophen hepatotoxicity and rescue: a mathematical model Theoretical Biology and Medical Modelling Springer Nature Link 05. Acetaminophen Toxicity Hospital Handbook Acetaminophen Overdose Reveals PAR4 as a Low Expressing but Potent Receptor on the Hepatic Endothelium in Mice Arteriosclerosis, Thrombosis, and Vascular Biology Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.